beta-2 Microglobulin 抗体
Quick Overview for beta-2 Microglobulin 抗体 (ABIN120759)
抗原
See all beta-2 Microglobulin (B2M) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- The antibody B2M-02 reacts with beta2-microglobulin (b2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta2-microglobulin.
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纯化方法
- Protein A chromatography
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纯度
- > 95 % (by SDS-PAGE).
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免疫原
- Purified isolated human beta2-microglobulin
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亚型
- IgG1
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anti-beta-2-Microglobulin (B2M) (AA 1-119) antibody
B2M 适用: 人 WB, ELISA, IF, IHC (p), RNAi, PLA 宿主: 小鼠 Monoclonal 3F9-2C2 unconjugated
anti-beta-2-Microglobulin (B2M) (AA 21-100) antibodyB2M 适用: 人 WB, FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 3G5H8 unconjugated
anti-beta-2-Microglobulin (B2M) (AA 22-119) antibodyB2M 适用: 小鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal A2 unconjugated
anti-beta-2-Microglobulin (B2M) (AA 22-119) antibodyB2M 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-beta-2-Microglobulin (B2M) antibodyB2M 适用: 人, Non-Human Primate FACS, IHC, IF, StM 宿主: 小鼠 Monoclonal rB2M-961 unconjugated Recombinant Antibody
anti-beta-2-Microglobulin (B2M) antibodyB2M 适用: 人, Non-Human Primate WB, FACS, IHC, IF, StM 宿主: 小鼠 Monoclonal B2M-961 unconjugated
anti-beta-2-Microglobulin (B2M) antibodyB2M 适用: 人, Non-Human Primate IHC, StM 宿主: 小鼠 Monoclonal B2M-1118 unconjugated
anti-beta-2-Microglobulin (B2M) antibodyB2M 适用: 人 WB, FACS, ELISA, IP, ICC, IHC (p), RIA 宿主: 小鼠 Monoclonal B2M-01 unconjugated
anti-beta-2-Microglobulin (B2M) antibody (Biotin)B2M 适用: 人 WB, FACS, ELISA, IP, ICC, IHC (p) 宿主: 小鼠 Monoclonal B2M-01 Biotin
anti-beta-2-Microglobulin (B2M) antibodyB2M 适用: 人, Non-Human Primate FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal B2M-961 unconjugated
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应用备注
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Flow cytometry (1 μg/mL): use PBL cell line as positive control. Negative control: DAUDI human lymphoma cell line, erythrocytes. Western blot (2 - 4 μg/mL): use U937 human lymphoma cell line as positive control. Sample preparation: Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer. Application note: Both reducing and non-reducing conditions. Reducing and non-reducing conditions. ELISA. Immunohistochemistry (Paraffin): Recommended dilution: 5 m g/mL, Positive tissue: liver
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 15 mM sodium azide, approx. pH 7.4
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch. -
有效期
- 12 months
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- beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))
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别名
- beta-2-Microglobulin
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背景
- B2M is a 12 kDa Ig like glycoprotein. B2M non-covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.Synonyms: B2M, Beta 2 microglobulin, Beta-2 microglobulin
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基因ID
- 567
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UniProt
- P61769
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途径
- TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
抗原
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